TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: AATHR    
Thrombophilia Profile, Plasma and Whole Blood

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Initial testing includes: prothrombin time (PT); activated partial thromboplastin time (APTT); dilute Russell viper venom time (DRVVT); thrombin time (bovine); fibrinogen; D-dimer; antithrombin activity; protein C activity; protein S antigen, free; prothrombin G20210A mutation; activated protein resistance V; and thrombophilia interpretation.

 

If PT is >13.9 seconds, PT mix will be performed at an additional charge.

 

If APTT is > or =38 seconds, APTT mix will be performed at an additional charge.

 

If APTT mix is > or =38 seconds and thrombin time is <35.0 seconds (no evidence of heparin), platelet neutralization procedure will be performed at an additional charge.

 

If DRVVT ratio is > or =1.20, DRVVT mix and DRVVT confirmation will be performed at an additional charge.

 

If thrombin time is > or =25.0 seconds, reptilase time will be performed at an additional charge.

 

If fibrinogen is <150 mg/dL, or clinically indicated, PT-Fibrinogen will be performed at an additional charge.

 

If D-dimer is >500 ng/mL FEU, soluble fibrin monomer will be performed at an additional charge.

 

If protein S antigen, free is <65% for males and females > or =50 years of age and <50% for females <50 years of age, protein S antigen, total will be performed at an additional charge.

 

If protein C activity is <70% with no evidence for an acquired decrease in protein C activity, protein C antigen may be performed at an additional charge.

 

If antithrombin activity is <80% with no evidence of an acquired decrease in antithrombin activity, antithrombin antigen will be performed at an additional charge.

 

If activated protein C resistance (APC) ratio is <2.3 or baseline APC APTT is prolonged, factor V leiden (R506Q) mutation analysis will be performed at an additional charge.

 

If appropriate, protein S activity, coagulation factor assays, or Staclot LA will be performed at an additional charge to clarify significant abnormalities in the screen test results.

 

If factor VIII result is <55%, the factor VIII inhibitor screen may be performed along with the Bethesda titering assay, if inhibitor screen is positive.

 

See Thrombophilia Profile in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Plasma Na Cit
Whole blood

Advisory Information

Multiple coagulation profile tests are available. See Coagulation Profile Comparison in Special Instructions for testing that is performed with each profile.

Shipping Instructions

Send all specimens in the same shipping container.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

See Coagulation Guidelines for Specimen Handling and Processing in Special Instructions.

 

Both blood and plasma are required.

 

Specimen Type: Whole blood

Container/Tube: 

Preferred: Yellow top (ACD)

Acceptable: EDTA, sodium citrate

Specimen Volume: 6 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Do not transfer blood to other containers.

3. Label specimen as whole blood.

 

Patient Preparation:

1. Patient should not be receiving Coumadin, heparin, direct thrombin inhibitors (argatroban, dabigatran), or direct factor Xa inhibitors (apixaban, rivaroxaban, and edoxaban).

2. Specimen must be drawn prior to initiation of anticoagulants and thrombolytic therapy.

Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Polypropylene vials

Specimen Volume: 5 mL in 5 Polypropylene vials each containing 1 mL

Collection Instructions:

1. Spin down, remove plasma, and spin plasma again.

2. Freeze plasma immediately (no longer than 4 hours after collection) at -20 degrees C or, ideally< or = -40 degrees C, .

3. Label specimens as plasma.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. If priority specimen, mark request form, give reason, and request a call-back.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Forms

1. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available in Special Instructions:

-Informed Consent for Genetic Testing (T576)

-Informed Consent for Genetic Testing-Spanish (T826)

2. Coagulation Patient Information (T675) in Special Instructions

3. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Whole Blood: 3 mL
Plasma: 5 mL in 5 polypropylene vials each containing 1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Plasma Na CitFrozen14 days
Whole bloodAmbient (preferred)7 days
 Frozen 14 days
 Refrigerated 14 days